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Recombinant Human Albumin Functions Equivalently to pHSA in Terms of Promotion of Cell Growth

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Recombinant Human Albumin Functions Equivalently to pHSA in Terms of Promotion of Cell Growth

Brand Name : Oryzogen
Model Number : HY100M1
Certification : ISO 9001 certificate
Place of Origin : China
MOQ : Negotiation
Price : Negotiation
Payment Terms : T/T
Supply Ability : 1000kg
Delivery Time : 3~5 days
Packaging Details : 1g/vial 10g/vial 50g/vial
Source : Rice Grain (Oryza Sativa)
Purity : Greater than 99%
Endotoxin : Less than 0.25EU/ug
Cell culture : CHO, iPS, ESC, vero, etc
Recommended concentration : 1~2g/L
Production Type : Recombinant Protein
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Quick Details

1. Source: Rice Grain (Oryza Sativa)

2. Physical Appearance: Off-white to light beige lyophilized powder (HY100M1)

3. Formulation: It is lyophilized with saline

4. Purity: More than 99% as determined by SDS-PAGE and HPLC analysis.

5. Endotoxin: Less than 0.125EU/mg


Description

Human serum albumin is the most abundant protein in human plasma. It can transport hormones, lipids and other molecules and maintain osmotic pressure. OsrHSA is a recombinant human serum albumin derived from rice grains, which provides an excellent solution for animal free medium. Compared to FBS, pHSA and BSA, OsrHSA has higher purity and excellent batch consistency. Meanwhile, OsrHSA is widely used as excipient, stabilizer and embedding agent in bio-pharmaceutical applications.

To test whether OsrHSA was functionally equivalent to pHSA in terms of promotion of cell growth, studies were performed with three common cell lines: Chinese hamster ovary (CHO),

Vero, and SP2/0. Treatment with either pHSA or OsrHSA (1 g/L) in DMEM containing 5% FBS dramatically increased the cell density and viability compared with control treatment (5% FBS only) in all cell lines. The levels of growth enhancement observed with OsrHSA and pHSA were nearly the same. The promotion of cell growth was comparable to that of CHO cells on 10% FBS. Specifically, OsrHSA showed a 20% increase in maximum viability and density compared with pHSA in Vero cells. Notably, the titer of antibody (IgG1+ κ) measured in the culture medium of SP2/0 cells supplemented with OsrHSA was significantly higher than that observed for the same cells supplemented with pHSA. Based on these results, we conclude that OsrHSA performed as well as pHSA in the promotion of cell growth, and thus can effectively replace pHSA in cell culture media.


Recombinant human serum albumin (rHSA) has good efficacy, such as rHSA has the same second and tertiary structure, physiological and biochemical properties with HAS from plasma. In addition, compared with HSA from plasma, rHSA has a lower immune source stimulation, and plays a better role in accelerating cell growth and metabolism, because rHSA is animal component free, which can avoid the prions or virus contaminants.

Recombinant human serum albumin produced in Oryza sativa (OsrHSA) has previously been documented to share similar physical and biochemical properties as plasma-derived human serum albumin. We therefore attempted to investigate in this report whether OsrHSA could serve as a potential replacement source for plasma-derived human serum albumin. We test medium contains 1mg/ml OsrHSA. Cells tested were split on day 0 and washed twice with phosphate buffered saline (PBS) the following day before switching to the testing medium or control medium.


Morphological assessment of hESCs cultured in 1mg/mL OsrHSA demonstrated healthy, compact colonies with minimal differentiation when compared to pHSA supplemented media.


Product Tags:

recombinant human albumin

      

humans serum albumin

      
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